CNT1 (SLC28A1)
Optivia Delivers FDA Ready Reports
QUESTIONS?
CNT1 Transporter: SLC28A1
About CNT1
The CNT1 transporter is a nucleoside concentrative transporter that is encoded by the SLC28A1 gene. CNT1 is expressed widely throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides. CNT1 is also localized in the blood brain barrier and can be responsible for hydrophilic compounds entering the brain.
CNT1 Substrates
CNT1 substrates include pyrimidine nucleosides and synthetic analogs such as zidovudine, gemcitabine and stavudine.
CNT1 Inhibition
CNT1 is inhibited by CNT1 substrates through competitive inhibition and non-substrates by other mechanisms.
Optivia CNT1 Transporter Assays
Optivia offers CNT1 substrate and inhibition assays in the gold standard model for transport: polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for CNT1 inhibitors and Vmax, Km and/or IC50 for CNT1 substrates.
The probe substrate for the Optivia CNT1 assay is uridine. Gemcitabine and adenosine serve as reference inhibitors. These serve as the positive controls for the CNT1 substrate assays and the CNT1 inhibition assays, respectively.
About CNT1
The CNT1 transporter is a nucleoside concentrative transporter that is encoded by the SLC28A1 gene. CNT1 is expressed widely throughout the body and plays an important role in nucleoside salvage pathways that lead to biosynthesis of nucleotides. CNT1 is also localized in the blood brain barrier and can be responsible for hydrophilic compounds entering the brain.
CNT1 Substrates
CNT1 substrates include pyrimidine nucleosides and synthetic analogs such as zidovudine, gemcitabine and stavudine.
CNT1 Inhibition
CNT1 is inhibited by CNT1 substrates through competitive inhibition and non-substrates by other mechanisms.
Optivia CNT1 Transporter Assays
Optivia offers CNT1 substrate and inhibition assays in the gold standard model for transport: polarized mammalian cells. We offer a wide range of studies including single concentration screens up to full kinetic studies to determine the IC50 for CNT1 inhibitors and Vmax, Km and/or IC50 for CNT1 substrates.
The probe substrate for the Optivia CNT1 assay is uridine. Gemcitabine and adenosine serve as reference inhibitors. These serve as the positive controls for the CNT1 substrate assays and the CNT1 inhibition assays, respectively.