More Transporters
in Better Models


Optivia Delivers FDA Ready Reports

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650-324-3177, extension 16
Toll Free: 888-OPTIVIA (888-678-4842)
Fax: 650-324-1855.




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Speak with one of our transporter specialists about your specific requirements to understand how we could help you and what kind of results yu can expect.

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Validated Transporter Assays from Optivia


Optivia transporter assays measure functional transport of a substrate in a polarized mammalian cell. Inhibition assays monitor the transport of a marker substrate and substrate transport assays measure transport of your compound.

Optivia has both efflux transporter assays and uptake transporter assays, including the seven FDA transporters in the ITC White Paper: P-gp, BCRP, OAT1, OAT3, OATP1B1, OATP1B3 and OCT2.

Optivia assays are validated with both reference substrates and inhibitors and must meet criteria for reproducibility.


Transporter

  Gene

   Cell
  Model

  Reference
  Substrate

   Reference
  Inhibitor

P-gp
(MDR1)

ABCB1
MDCK-II
 [3H]-digoxin verapamil

P-gp
(MDR1)

ABCB1
Caco-2
 [3H]-digoxin verapamil

BCRP
ABCG2
Caco-2
 [3H]-genistein chrysin

OCT1
SLC22A1
MDCK-II
 [14C]-metformin quinidine

OCT2
SLC22A2
MDCK-II
 [14C]-metformin quinidine

OAT1
SLC22A6
MDCK-II
 [3H]-p-aminohippurate probenecid

OAT3
SLC22A8
MDCK-II
 [3H]-estrone-3-sulfate probenecid

OATP1B1
SLCO1B1
MDCK-II
 [3H]-estrone-3-sulfate rifampicin

OATP1B3
SLCO1B3
MDCK-II
 [3H]-bromosulfophthalein rifampicin

NTCP
SLC10A1
MDCK-II
 [3H]-taurocholate glycocholate

CNT1
SLC28A1
MDCK-II
 [3H]-uridine gemcitabine